Cowboys coach Todd Payten has been forced into 14 days of isolation after being deemed a close contact of a positive COVID-19 case at the Townsville Markets on Sunday.
The Cowboys cancelled training on Wednesday after Payten was forced into isolation. He will be replaced by Dean Young who will act as interim head coach.
Cowboys chief executive Jeff Reibel said Payten visited the markets before the NRL enforced level four restrictions on players and staff.
Reibel said Tom Gilbert and Griffin Neame were also isolating after on Saturday travelling to Magnetic Island, which is now in lockdown.
Both players are free to return to training upon receiveing a negative result.
As a housemate of the duo, training squad member Laitia Moceidreke was also sent for a COVID-19 test and is in isolation awaiting results.
Strength and conditioning coach Mark Henry is also in isolation while awaiting the test result of a visiting family member, who was present on a flight to Townsville linked to a COVID-19 case.
All five players and staff in isolation are doing so away from their families.